Naslov
The Histone Chaperone Asf1 Increases the Rate of Histone Eviction at the Yeast PHO5 and PHO8 Promoters

Autori
Korber, Philipp ; Barbarić, Slobodan ; Luckenbach, Tim ; Schmid, Andrea ; Schermer, Ulrike J. ; Blaschke, Dorothea ; Hoerz, Wolfram

Izvornik
Journal of Biological Chemistry (0021-9258) 281 (2006); 5539-5545

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
chromatin remodeling; yeast PHO5 promoter; yeast PHO8 promoter; transcriptional regulation

Sažetak
Eukaryotic gene expression starts off from a largely obstructive chromatin substrate that has to be rendered accessible by regulated mechanisms of chromatin remodeling. The yeast PHO5 promoter is a well known example for the contribution of positioned nucleosomes to gene repression and for extensive chromatin remodeling in the course of gene induction. Recently, the mechanism of this remodeling process was shown to lead to the disassembly of promoter nucleosomes and the eviction of the constituent histones in trans. This finding called for a histone acceptor in trans and thus made histone chaperones likely to be involved in this process. In this study we show that the histone chaperone Asf1 increases the rate of histone eviction at the PHO5 promoter. In the absence of Asf1 histone eviction is delayed but the final outcome of the chromatin transition is not affected. The same is true for the coregulated PHO8 promoter where induction also leads to histone eviction and where the rate of histone eviction is strongly reduced in asf1 strains as well. Again, the final extent of chromatin remodeling is not affected. We also present evidence that Asf1 can work in concert with the SWI/SNF remodeling complex, but neither of the two cofactors are strictly dependent on each other.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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