Naslov
Artless and simple thin layer chromatography procedure for simultaneous identification and isolation of fumonisins

Autori
Jurišić, Blaženka ; Pepeljnjak, Stjepan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Interpretation of chemical, microbiological and biological results and the role of proficiency testing in accreditation of laboratories / Krauthacker B. ; Raspor B. - Zagreb : Institut za medicinska istraživanja i medicinu rada ; Institut Ruđer Bošković, 1998, S-06

Skup
5th International Symposium Chromatography and Hyphenated Techniques

Mjesto i datum
Varaždin, Hrvatska, 21.10.1998. - 23.10.1998

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
Fumonisins; TLC method; Fusarium moniliforme
(Fumonisins; TLC method Fusarium monilforme)

Sažetak
Fumonisins are naturally occuring toxic secondary metabolites of Fusarium moniliforme and other Fusarium species, thet may infect corn. Fumonisins are saturated long chain compounds that have no UV apsorbing chromophore, nor do they fluorescence. They contain a free primary amine and multiple freecarboxyl groups so minor changes in pH or ionic strenght in media, can remarkably affect on isolation and chromatographic behavior. In order to overcome these limitations, in this study we present a different, artless and simple approach. After the biosynthesis of an F. moniliforme culture in solid and liquid substrate media. acetonitrile/water mixture efficiently separate fumonisins. The water soluble phase, adjusted to pH 8-9 with NaHCO3, is then shaking with chloroform for subsequent purification. Water-soluble phase is concentrated in vacum by liophilisation. Solutions were spotted on preperative GF254 silica plates and develop with acetonitrile/toluene/water (93:3:2). Detection of FB1 is posible directly through florescence quenching in short UV light as bright blue zones.Confirmation of FB1 presence was made by IR method directly and byGC/MS method after hydrolisis and derivatisation with TMS reagent. In EIMS of the TMS derivates of the C-22 aminotetraols from hydrolisis products of FB1 backbones, the protonated molecular ions for the penta-TMS derivates and for tetra- TMS derivates as well as abundant fragments from cleavage between C-14 and C-15 were presented. Although these toxicants can never be completly removed from the food supply, it is necessery through risk assessment to define levels levels that are of health concern. The analytical method proposed here is quite accurate, more rapid, and less expensive than the other methods known. This may be useful in following the isolation of metabolites from extracts of Fusarium cultures as well as for efficient and simple isolation of fumonisin mycotoxins

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA