Naslov
Photoacoustic measurement of lutein in biological matrix

Autori
Bičanić, Dane ; Luterotti, Svjetlana ; Becucci, Maurizio ; Fogliano, Vincenzo ; Versloot, Pieter

Izvornik
Journal de physique. IV (1155-4339) 125 (2005); 825-828

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
photoacoustic spectroscopy; lutein; biological matrix

Sažetak
Photoacoustic (PA) spectroscopy was applied for the first time to quantify lutein in a complex biological matrix. Standard addition of lutein to a biological low-lutein matrix was used for the calibration. The PA signal was found linearly proportional (R > 0.98) to lutein concentration up to 0.3% (w/w). The dynamic range of concentrations extends to 1% (w/w) lutein. For a given experimental set-up the responsivity of PA detector within the range of linearity was estimated to 1.1 mV/1% lutein. Precision of repeated analyses is good with average RSD values of 4 and 5% for blanks and spiked samples, respectively. The analytical parameters indicate that the PA method is fast and sensitive enough for quantification of lutein in supplementary drugs and in the lutein-rich foods.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Farmacija

Napomena
Rad je prezentiran na skupu 13th International Conference on Photoacoustic and Photothermal Phenomena (ICPPP) održanom od 05.-08.07.2004.g., Rio de Janeiro, Brazil.

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