Naslov
Characterization of laboratory standards of tetanus toxin by polyacrylamide gel electrophoresis and western blott analysis

Autori
Ergotić, Neda ; Busch, Kristina ; Ballarin-Perharić, Alenka ; Čajavec, Stanislav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proc. 2 nd World congress of anaerobic bacteria and infections / Fr. Microbiol. Soc. - Nica : Characterization of laboratory standards of tetanus toxin by pol, 1998

Skup
2 nd World congress of anaerobic bacteria and infections

Mjesto i datum
Nica, Francuska, 03.10.1998. - 06.10.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
tetanus toxin; characterisation

Sažetak
Toxin of the C. tetani is used for immunisation of horses during the production of kyperimmune sera, as well as in the bioproductions of vaccines. to keep the quality of our products at high level it is necessary to control our laboratory standard of tetanus toxin regluarly. For this reason, the methods of our choise for characterisation of tetaunus toksin SDS-PAGE and Western blott analysis. In our study we have analysed lab. standard of C. tetani toksin in comparison with national standard. After visualization of the gel and nitrocellulose membrane it was noticed that about 2/3 of protein bands of the lab. standard of C. toxin and the national standard were coupled with specific horse immunoglobulins. Comparison of the standards shows that content of both standards does not differ significantlly.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA