Naslov
Analysis of the mouse transcriptome based on functional annotation of 60, 770 full-length cDNAs

Autori
Okazaki, Y. ; Furuno, M. ; Kasukawa, T. ; Adachi, J. ; Bono, H. ; Kondo, S. ; Nikaido, I. ; Osato, N. ; Saito, R. ; Suzuki, H. ; Yamanaka, I. ; Kiyosawa, H. ; Yagi, K. ; Tomaru, Y. ; Hasegawa, Y. ; Nogami, A. ; Schonbach, C. ; Lenhard, Boris

Izvornik
Nature (0028-0836) 420 (2002); 563-573

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
genom miša; transkriptom miša
(mouse genome; mouse transcriptome)

Sažetak
Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the mouse, and to ensure that each is represented in a physical collection of clones. Here we report the manual annotation of 60, 770 full-length mouse complementary DNA sequences. These are clustered into 33, 409 'transcriptional units', contributing 90.1% of a newly established mouse transcriptome database. Of these transcriptional units, 4, 258 are new protein-coding and 11, 665 are new non-coding messages, indicating that non-coding RNA is a major component of the transcriptome. 41% of all transcriptional units showed evidence of alternative splicing. In protein-coding transcripts, 79% of splice variations altered the protein product. Whole-transcriptome analyses resulted in the identification of 2, 431 sense-antisense pairs. The present work, completely supported by physical clones, provides the most comprehensive survey of a mammalian transcriptome so far, and is a valuable resource for functional genomics

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA