Naslov
Hydrocortisone and dexamethasone affect galectin-3 expression

Autori
Dabelić, Sanja ; Flögel, Mirna ; Dumić, Jerka

Izvornik
Periodicum biologorum (0031-5362) 107 (2005), 2; 175-181

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
hydrocortisone; galectin-3; dexamethasone; LGALS3

Sažetak
Background and Purpose: Galectin-3, a β -galactoside binding lectin, acts as a strong pro-inflammatory signal. Many immunomodulatory drugs affect signaling pathways that comprise transcription factors involved in regulation of galectin-3 gene (LGALS3) expression. The aim of our study was to investigate the effects of steroidal anti-inflammatory drugs (hydrocortisone - HC and dexamethasone - Dex) on the expression of galectin-3, both on the mRNA and the protein level. Materials and Methods: The human monocytic cell line THP-1 was exposed to various concentrations of HC (0.1, 1, 5 and 10 μ M) and Dex (0.1, 1, 10 and 100 nM) during 1, 3, 5, 24, 48 or 72 hrs. Relative RT-PCR method and GeneScan analysis software were used for assessing galectin-3 mRNA level and chemiluminescent-western blot analysis for measuring galectin-3 level. Results: The results showed that both drugs in all applied concentrations halved the galectin-3 mRNA level already after 1 h of exposure. The decrease continued and after 72 hours mRNA level dropped on 3.8-15.4 % of the initial value. The protein expression of galectin-3 didn’ t follow the same pattern ; although both drugs in all applied concentration provoked the reduction of galectin-3 level, the decrease was observed not until 48 and 72 hours of exposure, when galectin-3 level fell on 71 % to 38 % of the initial value, respectively. Conclusion: Steroidal anti-inflammatory drugs cause the reduction of both gene and protein expression of galectin-3, but the intensity of the changes and the time of their appearance strongly depends on the kind and the concentration of the drugs.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA