Naslov
Effects of aspirin and indomethacin on galectin-3

Autori
Dabelić, Sanja ; Flögel, Mirna ; Dumić, Jerka

Izvornik
Croatica chemica acta (0011-1643) 78 (2005), 3; 433-440

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
aspirin; galectin-3; indomethacin; LGALS3

Sažetak
Galectin-3, a β -galactoside binding lectin, acts as a strong pro-inflammatory signal. Many immunomodulatory drugs affect signaling pathways that comprise transcription factors involved in regulation of galectin-3 gene (LGALS3) expression. The aim of this study was to investigate the effects of non-steroidal anti-inflammatory drugs aspirin and indomethacin on the expression of galectin-3, both on mRNA and protein levels. The human monocytic cell line THP-1 was exposed to various therapeutic concentrations of aspirin and indomethacin for 72 hours. Relative RT-PCR method and the GeneScan analysis software were used for assessing the galectin-3 mRNA level and chemiluminescent-western blot analysis was used for measuring the galectin-3 level. The results showed that galectin-3 is a new target molecule of NSAIDs, which cause reduction of both gene and protein expression of galectin-3, but the intensity and time-course of the changes strongly depend on the kind and concentration of the drugs.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA