Naslov
Illegitimate integration of single-stranded DNA in Saccharomyces cerevisiae

Autori
Gjuračić, Krešimir ; Zgaga, Zoran

Izvornik
MGG. Molecular & general genetics (0026-8925) 253 (1996); 173-181

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
illegitimate recombination ; single-stranded plasmids ; saccharomyces cerevisiae

Sažetak
We studied illegitimate recombination by transforming yeast with a single-stranded (ss) non-replicative plasmid. Plasmid pCW12, containing the ARG4 gene, was used for transformation of yeast strains deleted for the ARG4, either in native (circular) form or after linearization within the vector sequence by the restriction enzyme ScaI. Both circular and linearized ss plasmids were shown to be much more efficient in illegitimate integration than their double- stranded (ds) counterparts and more than two- thirds of the transformants analysed contained multiple tandem integrations of the plasmid. Pulsed-field gel electrophoresis of genomic DNA revealed significant changes in the karyotype of some transformants. PLasmid DNA was frequently detected on more than one chromosome and on mitotically unstable, autonomously replicating elements. Our results show that the introduction of nonhomologous ss DNA into yeast cells can lead to different types of alteation in the yeast genome.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA