Naslov
Molecuar characterisation of B- and T- cell clonality in reactive and malignant lymphoid lesions
(Moleucar characterisation of B- and T- cell clonality in reactive and malignant lymphoid lesions)

Autori
Kušec, Rajko ; Bobetić-Vranić, Tanja ; Šiftar, Zoran ; Kardum-Skelin, Ika ; Dominis, Mara ; Jakšić, Branimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka / Jonjić, Nives ; Kardum-Skelin, Ika - Zagreb, 2005

Skup
3. Hrvatski kongres kliničke citologije s međunarodnim sudjelovanjem

Mjesto i datum
Opatija, Hrvatska, 08.05.2005. - 11.05.2005

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
molecular; clonality

Sažetak
Lymphoid cells proliferations, frequently clinically present as persistent lymphadenopathy and occasionally pose serious diagnostic problems in terms of distinguishing benign from malignant processes. In this context molecular analysis of B- and T-cell receptor rearrangements aimed at answering if the process is polyclonal, reactive non-malignant or clonal, accepted as neoplastic, can be useful. We present the results of molecular characterisation of B-cell receptor by PCR based assay for immunoglobulin heavy-chain gene CDR3 region and T-cell receptor by TCRγ multiplex PCR. Materials were 26 fine needle aspirates and 11 samples of paraffin embedded biopsies of lymph nodes from patients with persistent lymphadenopathy and inconclusive morphological and phenotypic diagnosis on the nature of lymphoid proliferation. Molecular findings of clonality were correlated to definitive cytological and histological diagnosis. Of 37 samples 16 were diagnosed as reactive lymphoid proliferations and 21 as malignant process, eg. lymphoma. For cytological samples (N=26) there was a good correlation of clonality with category of reacti...

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA