Pregled bibliografske jedinice broj: 21822
Differential gene expression in callus and tumour tissue lines of sugar beet (Beta vulgaris L.).
Differential gene expression in callus and tumour tissue lines of sugar beet (Beta vulgaris L.). // 1st Congress of Croatian Geneticists with International Participation : abstracts
Hvar, Hrvatska, 1998. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 21822 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Differential gene expression in callus and tumour tissue lines of sugar beet (Beta vulgaris L.).
Autori
Krsnik-Rasol, Marijana ; Čipčić, Hana ; Poljuha Danijela ; Hagege, Daniel
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
1st Congress of Croatian Geneticists with International Participation : abstracts
/ - , 1998
Skup
Congress of Croatian Geneticists with International Participation (1 ; 1998)
Mjesto i datum
Hvar, Hrvatska, 01.06.1998. - 04.06.1998
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
sugar beet; habituation; transformation; protein patterns; isoesterases
Sažetak
Normal, habituated and transformed in vitro tissue lines of sugar beet (Beta vulgaris L.) were studied with regard to patterns of cellular, extracellular proteins and isoesterases. A correlation between developmental processes and protein patterns was established. Electrophoretic patterns of cellular proteins were rather similar for all tissue lines. Silver stained gradient polyacrylamide gels showed few specific bands. The polypeptides of 16 and 17 kDa were found in normal and tumour tissue, the one of 37 kDa was tumour specific. The bands of 18 and 19 kDa were higher expressed in unorganised habituated tissue than in other tissue lines and the polypeptide of 23 kDa was characteristic for organised habituated tissue. In extracellular protein patterns differences were, at least quantitatively, more obvious. The polypeptides of 33, 23 and 13.5 kDa were not detected in extracellular matrix of unorganised habituated tissue, which contained more protein bands of higher molecular mass (54 or more kDa). This line is characterised by lack of cellulose and lignin in cell walls and reduced cell-to-cell adhesion (Tacchini P. et al., Plant Physiol. Biochem. 1995, 33/3/:361). The majority of extracellular proteins were glycosilated except the ones with molecular mass below 22 kDa. After incubation of the gels in solution containing 1-naphtylacetate and 2-naphtylacetate as substrates the isoesterases were visualised showing distinct patterns for different tissue lines.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
