Naslov
The influence of gastric pentadecapeptide BPC 157 on acute and chronic ethanol administration in mice. The effect of NG-nitro-L-arginine methyl ester and L-arginine

Autori
Boban-Blagaić, Alenka ; Blagaić, Vladimir ; Romić, Željko ; Jelovac, Nikola ; Dodig, Goran ; Ručman, Rudolf ; Petek, Marijan ; Turković, Branko ; Seiwerth, Sven ; Sikirić, Predrag

Izvornik
Medical science monitor (1234-1010) 12 (2006), 1; 36-45

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Gastric pentadecapeptide BPC 157; alcohol; acute intoxication; withdrawal; NO-agents

Sažetak
Alcohol disturbances, NO stimulation (by the NO-precursor L-arginine), and/or NO-synthesis blockade (by NG-nitro-L-arginine methyl ester, i.e. L-NAME) were challenged with stable gastric pentadecapeptide BPC 157, which inhibits both acute alcohol intoxication and alcohol withdrawal symptoms. Materials and methods. Mice received intraperitoneally (i.p.) BPC 157 (10 µ ; ; ; ; g/kg), L-NAME (10 mg/kg), and L-arginine (400 mg/kg), alone or in combination, 5 minutes before or after acute ethanol (4 g/kg i.p.) intoxication or after 0, 3, or 7 hours of withdrawal after drinking 20% alcohol for 13 days. Results. BPC 157 rapidly opposes the strongest disturbance presentations in acute intoxication (sustained ethanol anesthesia, complete loss of righting reflex, no reaction to external stimuli, hypothermia, 25% mortality) and withdrawal (prominent seizures). NO-agents: Aggravation of acute alcohol intoxication and opposition to withdrawal are common, but the later intervals affected by L-arginine and the action throughout the experiment by L-NAME are distinctive. Given together, L-arginine and L-NAME counteract each other, while either the “ L-NAME presentation” (acute intoxication) or the “ L-arginine presentation” (withdrawal) predominates. BPC157+NO-agent: In acute intoxication (L-NAME predominating in NO-system functioning to aggravate intoxication), both BPC157+L-NAME and BPC157+L-arginine follow the presentation of L-NAME, but without worsened mortality. In withdrawal (L-arginine predominating in NO-system functioning to oppose disturbance symptoms), BPC157+L-NAME follows the presentation of L-NAME, while BPC 157+L-arginine imitates that of L-arginine. Conclusion. The relationships among pentadecapeptide BPC 157, the NO-system, acute alcohol intoxication, and opposed withdrawal may be important, presenting pentadecapeptide BPC 157 as a suitable alcohol antagonist.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA