Naslov
Interaction of alcohols with serum LDL. An infrared study

Autori
Arrondo, Jose L.R. ; Coto, Xavier ; Milicua, Jose C.G. ; Kveder, Marina ; Pifat, Greta

Izvornik
Chemistry and Physics of Lipids (0009-3084) 141 (2006); 205-215

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
FTIR ; LDL ; pH dependence

Sažetak
The interaction of low molecular weight alcohols with low density lipoprotein (LDL) has been studied using amide I band-fitting, thermal profiling and two-dimensional infrared correlation spectroscopy (2D-IR). At 0.3 M alcohol, no changes in secondary structure are observed. In the presence of 1 M alcohol, ethanol and propanol decreases protein denaturation temperature and produces changes in the amide I thermal profiles of protein components and in the lipid bands. The 2D-IR synchronous map corresponding to protein or lipid component at 20– 37 °C suggests differences in the presence of propanol. The asynchronous map corresponding to the lipid component indicates changes in bandwidth, compatible with a more fluid environment. In the 37– 80 °C temperature range the thermal profile is different in the presence of propanol, both for the lipid and protein components. The results presented show that when alcohols affect the protein component, the lipid spectrum also varies pointing to an effect on the lipid-protein interaction.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

Napomena
Rad je rezultat COST D22 medjunarodne suradnje

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