Pregled bibliografske jedinice broj: 216011
New heavy labelled auxins for mass spectrometry
New heavy labelled auxins for mass spectrometry // Book of Abstracts, Second International Symposium "Auxins and Cytokinins in Plant Development" (Supplement to BIOLOGIA PLANTARUM, vol. 49 ; ISSN 0006-3134) / Čatský, Jiři (ur.).
Berlin: Springer, 2005. str. S28-S28 (poster, nije recenziran, sažetak, znanstveni)
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Naslov
New heavy labelled auxins for mass spectrometry
Autori
Buchtík, Roman ; Magnus, Volker ; Zatloukal, Marek ; Strnad, Miroslav
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of Abstracts, Second International Symposium "Auxins and Cytokinins in Plant Development" (Supplement to BIOLOGIA PLANTARUM, vol. 49 ; ISSN 0006-3134)
/ Čatský, Jiři - Berlin : Springer, 2005, S28-S28
Skup
Second International Symposium "Auxins and Cytokinins in Plant Development"
Mjesto i datum
Prag, Češka Republika, 07.07.2005. - 12.07.2005
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
heavy-isotope labelled auxin; amino acid conjugate; chemical synthesis; internal standard; mass spectrometry
Sažetak
The preparation of labelled derivatives of indole-3-acetic acid (IAA) is important for the standardization of mass spectrometry techniques. We are focused on the synthesis of IAA metabolites arising from [D5]-indole or [D5]-IAA as starting materials. In order to increase the number of isotopic atoms in the amino acid conjugates, N-15 labelled amino acids were used for the synthesis. The conjugates of [D5]-IAA with [N-15]-glycine, [N-15]-alanine, [N-15]-aspartic acid and [N-15]-glutamic acid were prepared by reaction of [D5]-IAA succinimide ester with the corresponding alpha-amino acid in alkaline medium. The aliphatic and aromatic N-15 labelled amino acids gave the conjugates via condensation of [D5]-IAA chloroformate anhydride with [N-15]-valine, [N-15]-leucine, [N-15]-isoleucine or [N-15]-phenylalanine in strongly alkaline medium. The same type of reaction was used for the preparation of [N-15]-indole-3-acetamide, where the mixed anhydride was poured into an aqueous solution of [N-15]-ammonia. Indole-3-ethanol and IAA-beta-D-glucosyl ester were labeled only in the indole ring. [D5]-indole-3-ethanol and [D5]-indole-3-methanol were prepared by reduction of either [D5]-IAA or [D5]-indole-3-carboxylic acid with lithium aluminium hydride.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija
POVEZANOST RADA
