Naslov
S-100 protein marker coexpressed on porcine gut immune cells is not orthologue of human CD8^+ CD11b^+ S-100^+ suppressor T lymphocytes

Autori
Lacković, Gordana ; Cvetojević, Maja ; Valpotić, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of the 4th International Meeting "Mechanisms in Local Immunity", Periodicum biologorum 100, Suppl 3 / Vitale, Branko - Zagreb : Hrvatsko prirodoslovno društvo, 1998, 79-79

Skup
The 4th International Meeting "Mechanisms in local immunity"

Mjesto i datum
Opatija, Hrvatska, 16.09.1998. - 19.09.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Gut-asociated lymphiod tissue; porcine; S-100 protein; immunochistochemical staining; suppressor T lymphocytes.

Sažetak
We have demonstrated the expression of CD3a and S-100 protein molecules on porcine immune cells populating the gut-associated lymphoid tissues (GALT). The distribution patterns of CD3a^+ and S-100 protein^+ cells within GALT compartments, such as jejunal and ileal lamina propria (JLP and ILP), and ileal Peyer’s patches (IPP) of juvenile 8-week-old, conventionally reared pigs were examined using double staining immunohisichemical methods. The samples of jejunum and ileum were fixed in methanol-chloroform-acetic acid and embedded in paraffin and processed for immunohistochemical staining. The S-100 protein marker was detected by polyclonal antibodies (pAbs) using peroxidase-antiperoxidase (PAP) staining, and subsequently, the CD3a antigen was identified by monoclonal antibodies (mAbs) using avidin-biotin complex (ABC). The use of rabbit anti-bovine S-100 protein pAbs, in the PAP method, revealed numerous mononuclear cells around the crypts of JLP and ILP, and in the IPP. Solitary S-100 protein^+ cells were present among capillary endothelial cells of JLP and ILP. In the ABC method, using mAbs against CD3a epitope of porcine T cells, we have found that the most numerous CD3a^+ cells were intraepithelial lymphocytes and T cells between IPP. These cells were rarely distributed in the lamina propria and practically absent in the crypts. Double positive, CD3a^+ S-100 protein^+ T cells were sporadically (if any) demonstrable in the ILP, and thus, their assignment as porcine orthologue of the human CD8^+ CD11b^+ S-100 protein^+ suppressor lymphocytes seems to be unlikely. Hence, their role in down regulation of porcine intestinal immune responses still remains to be definitely established.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA