Naslov
Immunogenic PGDF-AB dimers in crude leucocyte interferon batches

Autori
Židovec, Snježana ; Mažuran, Renata

Izvornik
Cytokine (1043-4666) 10 (1998), 1; 70-73

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Sažetak
The infection of human peripheral blood leukocytes (hPBL) with Sendai virus in vitro, induces the synthesis of human leukocyte interferons (IFN-alpha n3) as well as other cytokines. The authors discovered that supernatants of virus-infected hPBL also contained significant amounts of platelet-derived growth factor AB (PDGF-AB). The concentrations of immunogenic PDGF-AB in the interferon batches were determined by quantitative enzyme-linked immunosorbent assay (ELISA) before and after the inactivation of Sendai virus by acidification (pH 2) for 5 days. Immunogenic PDGF-AB molecules were detected in all interferon batches before and after acidification. The virus inactivation process caused a significant change in the content of immunogenic PDGF-AB in the interferon samples. Inactivation of the Sendai virus by acidification favoured the appearance of PDGF-AB dimers in the majority of samples.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA