Pregled bibliografske jedinice broj: 214610
Primjena FICTION tehnike na rutinski fiksiranim uklopljenim u parafin bioptičkim uzorcima koštane srži
Primjena FICTION tehnike na rutinski fiksiranim uklopljenim u parafin bioptičkim uzorcima koštane srži // 3rd Croatian Congress of Pathology and Forensic Medicine, Book of Abstracts
Opatija, Hrvatska, 2005. (predavanje, nije recenziran, sažetak, znanstveni)
CROSBI ID: 214610 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Primjena FICTION tehnike na rutinski fiksiranim uklopljenim u parafin bioptičkim uzorcima koštane srži
(Application of the FICTION technique on routinely fixed paraffin wax-embedded bone marrow trephines)
Autori
Korać, Petra ; Jones, M ; Dominis, Mara ; Kušec, Rajko ; Mason, DY ; Banham, AH ; Ventura, RA
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
3rd Croatian Congress of Pathology and Forensic Medicine, Book of Abstracts
/ - , 2005
Skup
3rd Croatian Congress of Pathology and Forensic Medicine
Mjesto i datum
Opatija, Hrvatska, 08.05.2005. - 11.05.2005
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Nije recenziran
Ključne riječi
FICTION tehnika
(FICTION technique)
Sažetak
Aim: We report a new application of FICTION technique to simultaneously detect both immunophenotype and chromosomal abnormalities in routinely fixed paraffin wax-embedded bone marrow trephines. Methods: Methods that we used were FISH (used probes were commercial) and immunostainig with antibody against CD 20 that is commonly used in routine analyses. Results: The FICTION technique was applied to BMTs that had been decalcified with either an ETDA-based or an HCl-based reagent. Attempts to perform FICTION on acid-decalcified BMTs (n=6) had adequate immunostaining but no FISH signals. In contrast, the EDTA based treatment enabled the successful application of combined immunohistochemistry and FISH. The FISH signals in each of the cases (n=16) were bright, focused and easily interpretable using a x63 objective lens and the immunofluorescent staining in each sample was bright, specific, did not fade and facilitated the rapid identification of neoplastic cells. Conclusion: For the first time we report the successful application of a modified FICTION technique to allow the study of BMTs. This technique allows the rapid identification of rare CD20-positive B-cells within the bone marrow by immunophenotyping as well as simultaneously identifying any specific genetic aberrations harboured by these cells.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
