Pregled bibliografske jedinice broj: 214505
The development of a mouse mutant system for studying the role of NKG2D in vivo
The development of a mouse mutant system for studying the role of NKG2D in vivo // Annual meeting of the Croatian Immunological Society, 2005 / Jonjić, Stipan (ur.).
Rijeka: Hrvatsko imunološko društvo, 2005. (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 214505 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The development of a mouse mutant system for studying the role of NKG2D in vivo
Autori
Zafirova, Biljana ; Antulov, Ronald ; Polić, Bojan
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Annual meeting of the Croatian Immunological Society, 2005
/ Jonjić, Stipan - Rijeka : Hrvatsko imunološko društvo, 2005
Skup
Annual meeting of the Croatian Immunological Society, 2005
Mjesto i datum
Božava, Hrvatska, 29.09.2005. - 02.10.2005
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
NKG2D; NK cells; Cre/loxP technology; EGFP
Sažetak
NKG2D is an activating receptor expressed on NK, NKT and some subpopulations of T cells (i.e. CD8+ TCRab+ activated and memory T cells, TCRgd+ T cells). It belongs to the C-type lectin-like family of receptors and interacts with stress-induced H60, Rae1 and Mult1 ligands that can be expressed on various cell types. The aim of this work is to generate a mouse mutant system to study the role of NKG2D in the development, activation, and homeostasis of the NKG2D expressing cell types in vivo. Therefore, we have been developing two NKG2D mutant mouse strains: NKG2D- EGFP knock out/in and NKG2D conditional knock out. The NKG2D-EGFP mutation will serve to trace the NKG2D expressing cells and to achieve the conventional inactivation of the gene in the same time. In the NKG2D conditional knock out mouse will be possible to inactivate the gene in a conditional manner, using the Cre/loxP system, to study its role in specific cell types (NK, NKT and T cells) and at the defined time. In order to achieve above mentioned mutations we designed two targeting vectors and made NKG2D gene targeting in Bruce 4 (C57BL/6) embryonic stem (ES) cells. Upon the selection and screening procedure we obtained several homologous recombinants from each targeting. The NeoR deleted ES clones where then microinjected and we obtained several chimeric mice of each injected clone.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
