Naslov
In search for genotoxicity: fish

Autori
Klobučar, Goran, Igor, Vinko ; Štambuk, Anamaria ; Pavlica, Mirjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts, Biomarkers, Risk and Assessment / Hylland, Ketil - Oslo : Central and Eastern Europe Programmes, The Research Council of Norway, 2005

Skup
CroWat final conference

Mjesto i datum
Cavtat, Hrvatska, 06.04.2005. - 10.04.2005

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Genotoxicity; Biomonitoring; Fish

Sažetak
Genotoxicity monitoring of marine and freshwater environment was conducted as a part of the three-year CROWAT project. DNA damage was evaluated in erythrocytes of fish collected from their natural habitat (Mullus barbatus) as well as from caged fish (Dicentrarchus labrax and Cyprinus carpio). DNA integrity of fish blood cells was analysed by comet and micronuclei (MN) assays. While the alkaline comet assay detects primary repairable, therefore reversible DNA lesions (alkali labile sites, single strand DNA breakages), the MN assay detects more persistent DNA lesions or aneugenic effects that cannot be repaired. Persistence of MN in the cell is therefore longer than single strand DNA breakages and alkali labile sites that are being, more or less constantly repaired. The benefit of the combined use of these two assays in genotoxicity monitoring of Croatian marine and freshwater environment will be discussed.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA