Naslov
RecBCD Enzyme Overproduction Impairs DNA Repair and Homologous Recombination in Escherichia coli

Autori
Đermić, Damir ; Halupecki, Edyta ; Zahradka, Davor ; Petranović, Mirjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts. Second Congress of Croatian Geneticists with International Participation / Franekić Čolić, J. ; Ugarković, Đ. - Zagreb : Hrvatsko genetičko društvo, 2005, 88-88

Skup
Second Congress of Croatian Geneticists with International Participation

Mjesto i datum
Supetar, Hrvatska, 24.09.2005. - 27.09.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Escherichia coli; RecBCD enzyme; DNA repair; homologous recombination

Sažetak
The Escherichia coli RecBCD enzyme is a powerful helicase and nuclease that processes DNA molecules containing blunt double-strand DNA end. Mutants deprived of RecBCD enzyme functions are extremely sensitive to DNA-damaging agents, poorly viable and severely deficient in homologous recombination. Remarkably, such important cellular functions rely on only about 10 molecules of RecBCD present in a cell. To determine the effect of an increased concentration of RecBCD enzyme and its derivatives on cellular processes that depend on the enzyme, we introduced wild-type and mutant alleles of recBCD genes on a low-copy-number plasmid into recB and wild-type bacteria and assessed their capacity for DNA repair and homologous recombination. We found that the overproduction of RecBCD enzyme, as well as RecBC and their nuclease-deficient derivatives, impairs both DNA repair and homologous recombination in E. coli. Our collective results suggest that DNA binding and processive helicase activities of the overproduced RecBCD enzyme, or its derivates, impair DNA repair and homologous recombination in E. coli. The cells control these activities of RecBCD by maintaining its extremely low concentration, thereby allowing efficient DNA repair and homologous recombination.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA