Naslov
Genetic diversity in European perennial ryegrass cultivars investigated with RAPD markers

Autori
Bolarić, Snježana ; Barth, Susanne ; Melchinger, E. Albrecht ; Posselt, K. Ulrich

Izvornik
Plant breeding (0179-9541) 124 (2005), 2; 161-166

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lolium perenne; RAPD marker; diversity; cultivars; Rogers' distance; minimum sample size

Sažetak
Perennial ryegrass (Lolium perenne L.) is the most important grass species for temperate grassland agriculture. The genetic relationship and distance among cultivars is largely unknown but of great interest for breeding programmes. The objectives of this study were to (i) investigate the molecular variation and structure of population cultivars, (ii) describe the relationship among cultivars in terms of the modified Rogers' distance, and (iii) determine the minimum sample size required for characterization of cultivars of L. perenne using random amplified polymorphic DNA (RAPD) markers. A total of 22 ryegrass cultivars, mainly of European origin, were investigated with RAPD markers. The minimum sample size required for the characterization of cultivars was about 20 individuals per population. An analysis of molecular variance (amova) revealed a much larger genetic variation within cultivars (66%) than between them (34%).

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija

POVEZANOST RADA