Naslov
Chromosome abnormalities in neuroblastzoma: cytogenetic, cytologic and FISH analysis

Autori
Nakić, Melita

Izvornik
Medical and pediatric oncology (0098-1532) 39 (2002), 4; 335-335

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kongresno priopcenje, znanstveni

Ključne riječi
Cytogenetics; Cytology; FISH; neuroblastoma

Sažetak
Neuroblastoma is one of the most common solid tumors in childrern. Cytohgenetic analysis revealed recurrent chromosome aberrations. The chromosome rearrangements important in tumor evaluation are near diploid or tetraploid chromosome number, terminal deletion of the short arm of chromosome No 1, double minutes, homogeneously staining regions and amplification of the N-myc protooncogene. These genomic imvbalances contribute to the tumor phenotype and carry useful prognostic information. In this report the results of cytogenetic aalysis in 10 children with neuroblastoma are reported. The aim of this study is to identify numerical and structural aberrations and determine the frequency and types of acquired genomic abnormalities in our group of children with neuroblastoma. Cytogenetic investigation was performed on slides obtained by direct method of tumor tissue treatment. GTG- and CBG- banding method were used for chromosome identification. FISH analysis was carried out using locus specific and chromosome specific centromeric probes (Vysis). The analysis of malignant cells revealed chromosome abnormalities in 9 cases, while in one tumor normal diploide karyotype was identified. Most tumor present near- diploid or tetraploid chromosome number while line was near triplod. Abberations of chromosome No 1 where the most frequent clonal arrangements including translocatzions, terminal deletions, duplications and isochromosome. N-myc amplification was detected by cytogenetic and FISH analysis in one patient. This study confirms cytogenetic heterogeneity of neuroblastoma in correlation to cytomorphological diagnosis and prognosis of the disease, advantages of FISH, especially interphase FISH in the detection of genomic changes in neuroblastoma.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA