Naslov
Rat fetal neural retina cultivated at ectopic sites

Autori
Bulić-Jakuš, Floriana ; Jurić-Lekić, Gordana ; Katušić, Ana ; Jovanov-Milošević, Nataša ; Vlahović, Maja ; Šerman, Ljiljana ; Ježek, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of 7th Multinational Congress on Microscopy / Čeh, Miran ; Dražič, Goran ; Fidler, Sanja - Ljubljana : Slovene Society for Miroscopy, 2005, 439-440

Skup
7th Multinational Congress on Microscopy

Mjesto i datum
Portorož, Slovenija, 26.06.2005. - 30.06.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Neural retina; rat; organ culture

Sažetak
Investigations of the developmental potential of different eye structures such as the retina are important in designing novel approaches to tissue replacement therapy aimed to cure damaged parts of the eye. The purpose of here presented experiments was to further investigate the developmental potential of the fetal rat retina cultivated in vitro or transplanted in vivo at an ectopic site. Neural retinas were microsurgically isolated from 20-days-old Fischer rat fetuses under the dissecting microscope. They were cultivated in an organ culture system at the air-liquid interface for 12 days in vitro in protein-free defined MEM supplemented with 50% rat serum or 50 ug/ml transferrin. Explants were fixed in St Marie's solution (1% acetic acid in 96% ethanol, +40C), dehydrated and embedded in paraffin. Uninterrupted serial sections (5  m) were used for histological analysis. Adult Fischer males were anaesthetized with ether and the skin and muscle cut to approach the kidney. A small “ pocket” was done under the kidney capsule to place the transplant where it spent 120 days. Specimens were fixed in 4% buffered glutaraldehyde and postfixed in 1% OsO4. After dehydration, embedding in Durcopan was done and ultra-thin sections were contrasted with lead cytrate and uranyl acetate and examined by transmission electron microscopy. Fetal rat retina survived in in vitro explants although the normal tissue architecture was lost and rosettes were formed. By electron microscopy all main types of neural and glia cells could be recognized in explants. Photoreceptors could be distinguished by the cilium and their characteristic outer segment containing flattened discs parallel one to the other but perpendicular to the outer limiting membrane. Moreover a photoreceptor in the process of shedding its outer segment was found. In transplants retinal tissue also survived for 120 days and formed rosettes. By electron microscopy neural and glia cells with abundant neuropil as well as plexiform layers were found. However, typical photoreceptors have not yet been found. By this study we confirmed our previous results that fetal neural retina of the rat can survive in vitro in a three-dymensional organ culture model in serum-supplemented and chemically defined culture medium, although the normal tissue architecture is lost. However, retinal cells seem to have retained their typical morphology and even the physiological process of photoreceptor outer segment shedding could take place. Rat fetal retina survived in ectopic transplants in vivo for 120 days which was two and half months more than we previously described.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA