Naslov
Free fatty acid level after experimental spinal cord injury

Autori
Pantović, Radojka ; Eraković, Vesna ; Blagović, Branka ; Draganić, Pero ; Simonić, Ante ; Milin, Čedomila

Izvornik
Chemistry and physics of lipids (0009-3084) 118 (2002), 1-2; 104-105

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kongresno priopcenje, znanstveni

Ključne riječi
free fatty acids; spinal cord injury; rabbits

Sažetak
Fatty acids are essential components of membrane phospholipids having therefore an important role in maintaining of a structure and a function of cell membranes. Accumulation of free free fatty acids (FFA), especially arachidonic acid, precursor of prostaglandins and leukotriens, is a possible indicator of nervous cell damage. In this study we have followed the effect of impact spinal cord injury on the level of free palmitic, stearic, oleic, arachidonic and docosaheksaenoic acid in an experimental model of spinal cord injury in rabbits. Spinal cord samples from the impact injury site were taken and frozen in liquid nitrogen. Modified method by Folch, for the isolation and purification of total lipids, was used. The FFAs were separated from the total lipid extract by preparative thin-layer chromatography. Free fatty acids was identified and quantified using gas chromatography. The level all of research free fatty acids was increased in the spinal cord of experimental animals after neurotrauma.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti

POVEZANOST RADA