Naslov
A clinoptilolite effect on cell media and the consequent effects on tumor cells in vitro

Autori
Katić, Maša ; Bošnjak, Berislav ; Gall-Trošelj, Koraljka ; Đikić, Ivan ; Pavelić, Krešimir

Izvornik
Frontiers in bioscience (1093-9946) 11 (2006); 1722-1732

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
clinoptilolite; media; cell signaling; apoptosis

Sažetak
Clinoptilolite is a nontoxic natural zeolite with properties of an ion-exchanger and adsorbent. Earlier studies showed that clinoptilolite could be an adjuvant in cancer therapy. The aim of this study was to define effects of clinoptilolite in cell media in cell viability and activity of key proteins regulating cell survival, cell division and stress response. The number of viable cells, DNA synthesis and activity of EGF-R, PKB/Akt and NF B was reduced, while apoptosis was increased in cells that were cultured in medium supplemneted with clinoptilolite. These results might be due to adsorbtion of some serum components such as EGF to clinoptilolite. In treated medium without serum the predominant role of clinoptilolite is that of cation exchange, likely affecting calcium levels and calcium-dependent signalling pathways. These results are in line with other data that confirm enhanced apoptosis in cells incubated in treated medium. Together, data presented here demonstrate that clinoptilolite affects cellular microenvironment through mechanisms that are dependent on adsorptive and ion-exchange characteristics of this material.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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