Naslov
Intimate association of Thy-1 and the T-cell antigen receptor with the CD45 tyrosine phosphatase

Autori
Volarević, Siniša ; Burns, Christopher M. ; Sussman, Jeffrey J. ; Ashwell, Jonathan D.

Izvornik
Proceedings of the National Academy of Sciences of the United States of America (0027-8424) 87 (1990), 18; 7085-7089

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
CD45 tyrosine phasphatase ; TCR ; Thy-1

Sažetak
Immunoprecipitation of Thy-1 from Triton X-100 detergent lysates of surface-iodinated and chemically cross-linked T cells precipitated at least five major and discrete bands. Four of these bands were identified as Thy-1, CD45 (a transmembrane tyrosine phosphatase), a major histocompatibility complex-encoded class I molecule, and beta 2-microglobulin. Similar analyses revealed that CD45 was coprecipitated from lysates of cross-linker-treated cells by antibodies to the T-cell antigen receptor (TCR). The same pattern of coprecipitated bands was observed when digitonin was used to lyse untreated cells. Immunoprecipitation of Thy-1 or the TCR from lysates of cross-linked T cells precipitated CD45 tyrosine phosphatase activity. Calculations based upon the amounts of coprecipitated enzymatic activity or TCR zeta chain indicate that a substantial fraction of Thy-1 and TCR complexes can be cross-linked to CD45. These data support a model in which the dependence of Thy-1 signaling on TCR coexpression is due to their common interaction with a tyrosine phosphatase and provide a possible structural basis for the influence of CD45 on TCR-mediated signaling.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA