Naslov
Ig heavy gene rearrangement in lymphoid neoplasia - a molecular biology approach

Autori
Kušić, Borka ; Dominis, Marija ; Džebro, Sonja ; Katičić, Miroslava ; Antica, Mariastefania

Izvornik
40 godina molekulane biologije u Hrvatskoj

Vrsta, podvrsta
Ostale vrste radova, popularni rad

Godina
1998

Ključne riječi
PCR; Ig heavy chain gene rearrangement; B lymphocytes

Sažetak
We selected the appropriate and reliable molecular biology method to provide a unique marker for detecting a monoclonal B lymphocyte population, which allows us to distinguish a monoclonal versus a polyclonal rearrangement of the IgH gene in B cells. We applied a semi-nested PCR based on: - germline state (in all cells except B lineage lymphocytes) V and J segments of IgH gene are several thousands bases apart and therefore cannot be amplified by the selected primers - during B lymphocyte differentiation V and J segment are brought close together and can be amplified by PCR using specific primers The method is especially useful in cases: - when conventional methods do not give a conclusive diagnosis - when there is limited supply of tissue (MALT)

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA