Naslov
Database for PKSS and NRPSS: computer programs to automatically find and annotate new sequences

Autori
Diminić, Janko ; Zavišek, Karolina ; Široka, Vilson ; Radulović, Žarko ; Starčević, Antonio ; Hranueli, Daslav ; Cullum, John

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final Programme and Book of Abstracts / Franekić Čolić, Jasna ; Ugarković, Đurđica - Zagreb : Hrvatsko genetičko društvo, 2005, 69 (PI-8)

Skup
Second Congress of Croatian Geneticists with international participation

Mjesto i datum
Supetar, Hrvatska, 24.09.2005. - 27.09.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
DNA sequencing projects; PKS and NRPS modular clusters; EMBL database; BLAST; genes; modules; domains; cluster annotation

Sažetak
Recent DNA sequencing projects (e.g. genome sequences) are yielding a large number of new potential PKS and NRPS modular clusters whose functionality and products are unknown. A program has been written to scan the EMBL database using BLAST to identify potential clusters. This program is run regularly and used to identify new clusters that have been added to the EMBL database. The new clusters are downloaded and screened for potential modules using further programs. The output is then screened by eye to decide if a complete cluster is likely to be present. Further programs analyse these potential clusters to identify domains. By using models for the structure of typical modules it is possible in most cases to assemble the domains into modules. Operator intervention is necessary if atypical module structures occur. The final program enters the newly annotated clusters into the database. Clusters are only entered after a human decision that the annotation is appropriate. This system will allow the rapid addition of novel sequences that have not been adequately annotated previously. There will be a computer demonstration of programs.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA