Naslov
Solid-phase extraction and TLC quantification of enrofloxacin, oxytetracycline, and trimethoprim in wastewater

Autori
Ašperger, Danijela ; Mutavdžić, Dragana ; Babić, Sandra ; Horvat, Alka J.M. ; Kaštelan-Macan, Marija

Izvornik
Journal of planar chromatography, modern TLC (0933-4173) 19 (2006), 108; 129-134

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
veterinary antibiotics ; enrofloxacin ; oxytetracycline ; trimethoprim ; water ; solid-phase extraction (SPE) ; thin-layer chromatography (TLC)

Sažetak
Quantitative determination of antibiotics enrofloxacin, oxytetracycline and trimethoprim in aquatic samples are reported. Method involves solid-phase extraction (SPE) and preconcentration of antibiotics, separation of samples by means of thin-layer chromatography (TLC) and quantification by video densitometry. TLC separation was performed on HPTLC CN F254s plates with H2C2O4 - methanol as the mobile phase. Spots were detected and quantified at 254 nm by videodensitometry. Limits of detection (LOD) and quantification (LOQ) for TLC determination were calculated. The method was examined on spiked water samples. Antibiotics were extracted by solid-phase extraction (SPE) procedure using polystyrenedivinylbenzene (SDB) Empore extraction disk. The extraction efficiency was checked by recovery experiments.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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