Naslov
Determination of veterinary pharmaceuticals in production wastewater by HPTLC-videodensitometry

Autori
Babić, Sandra ; Mutavdžić, Dragana ; Ašperger, Danijela ; Horvat, Alka J. M. ; Kaštelan-Macan, Marija

Izvornik
Chromatographia (0009-5893) 65 (2007), 1-2; 105-110

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
thin layer chromatography ; videodensitometry ; veterinary pharmaceuticals ; wastewater

Sažetak
An SPE-HPTLC method for simultaneous identification and quantification of seven pharmaceuticals in production wastewater was optimized and validated. The studied compounds were enrofloxacine, oxytetracycline, trimethoprim, sulfamethazine, sulfadiazine, sulfaguanidine and penicillin G/procaine. The method involves solid-phase extraction on hydrophilic-lipophilic balance cartridges with methanol and HPTLC analysis of extracts on CN modified chromatographic plates followed by videodensitometry at 254 and 366 nm. Optimization of chromatographic separation was performed by systematic variation of the mobile phase composition using genetic algorithm approach and the optimum mobile phase composition for TLC separation was 0.05 M H2C2O4:methanol = 0.81:0.19 (v/v). Linearity of the method was demonstrated in the ranges from 1.5 to 15.0 lg L)1 for enrofloxacine, 100– 500 lg L)1 for oxytetracycline, 150-600 lg L)1 for trimethoprim, 300-1100 lg L)1 for sulfaguanidine and 100-400 lg L)1 for sulfamethazine, sulfadiazine and penicillin G/procaine with coefficients of determination higher than 0.991. Mean recoveries ranged from 74.6 to 117.1% and 55.1 to 108.0% for wellspring water and production wastewater, respectively. Only sulfaguanidine showed lower results. The described method has been applied to the determination of pharmaceuticals in wastewater samples from pharmaceutical industry.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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