Naslov
Oxidases as enzymes for potential industrial purposes

Autori
Findrik, Zvjezdana ; Vasić-Rački, Đurđa

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Enzyme Reaction Engineering: Book of Abstracts, 2nd Croatian-German Conference / Vasić-Rački Đurđa ; Zelić, Bruno - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2005, 70-72

Skup
Enzyme Reaction Engineering, 2nd Croatian-German Conference

Mjesto i datum
Cavtat, Hrvatska, 21.09.2005. - 24.09.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
amino acid oxidase; modelling; enzyme kinetics

Sažetak
Increasing number of oxidative biotransformations is being reported indicating wide variability in the biocatalyst characteristics and a range of potential and established applications. If these biotransformations are to be accepted in industry, they should include improvements like integration with chemical processes and downstream processes1. One of the important requirements for a successful biocatalytic process is the availability of the enzyme in adequately large amounts2. Once this problem is resolved, more industrial processes with oxidases should be expected. The advantage of oxidizing enzymes in comparison to chemical catalysis is in their high redox-potential which enables them to catalyze reactions with chemically stable reactants, and in the predictability of the reaction products. A great disadvantage of processes that include oxidases is cofactor NAD(P)H requirement (especially in the case of dehydrogenases), which is very expensive and should be regenerated in order to make the process profitable. Another type of oxidases are FAD depending and they don’ t require special cofactor regeneration but they produce hydrogen peroxide. FAD re-oxidation depends on oxygen, which acts as electron-acceptor and is reduced to hydrogen peroxide during the reaction. Amino acid oxidases belong to that group of FAD-depending enzymes. They are weakly described in the literature even though they are commercially interesting. Nowadays there are many innovative cofactor NAD(P)H regeneration systems reported3 that have been developed using dehydrogenase systems, electrochemical regeneration and photoreduction. Besides these regenerating systems, chemical engineering methods resolve enzyme’ s problems in other areas like instability and low activity. The greatest problem for oxidases application in industry is small number of low price oxidizing enzymes. Nevertheless, some of them have found their application in large scale processes like: in the production of phenols, cis-hydrodiols, halogenated hydrodiols, catechols, L-tert-leucin and cycloalkanols. Products of oxidase processes also include hydroxyl nicotinic acid, hydroxyphenoxypropionic acid etc.4 Lacasses and peroxidases are being used in textile industry for dye removal and they are also used in bioremediation processes. Amino acid oxidases have also found their role in commercialized bioprocesses like in the production of 7-ACA and the production of enantiopure aminoacids5. They also have a great potential for commercial application in biosensors since this area is greatly investigated.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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