Naslov
Evaluation of antioxidant activity by flow injection analysis method with electrochemically generated ABTS radical cation

Autori
Iveković, Damir ; Milardović, Stjepan ; Roboz, Mario ; Grabarić, Božidar

Izvornik
Analyst (0003-2654) 130 (2005), 15; 708-714

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
antioxidant activity ; abts radical cation ; flow injection ; Trolox

Sažetak
A Trolox equivalent antioxidant capacity (TEAC) decolourisation assay was adapted to a flow injection analysis (FIA) system and a simple and rapid method for antioxidant activity evaluation was developed. To avoid the time consuming step of 2, 2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation preparation by chemical oxidation of ABTS, as in the original TAEC assay, and hence, to shorten an analysis time, the ABTS radical cation was generated on- line by electrochemical oxidation of ABTS in the flow-through electrolysis cell forming a part of the FIA system. The proposed method was optimised with respect to a flow rate, injection volume and ABTS radical cation / carrier ratio. Under the optimised conditions linear calibration graphs for Trolox were obtained over the range 10 - 100 mM, with a limit of detection of 1.6 mM. Good reproducibility (relative standard deviation of 1.95 %) and sample throughput (32 samples per hour) were achieved. The developed method was applied to the evaluation of antioxidant activity of pure compounds and samples of some common beverages. In both cases a good correlation between the results obtained by the proposed method and TEAC values evaluated by the classic TAEC decolourisation assay was obtained (r2 = 0.996 for pure compounds and r2 = 0.957 for beverage samples).

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA