Naslov
The secondary cell wall polymer of Geobacillus tepidamans GS5-97(T): structure of different glycoforms

Autori
Steindl, Christian ; Schäffer, Christina ; Smrečki, Vilko ; Messner, Paul ; Müller Norbert

Izvornik
Carbohydrate research (0008-6215) 340 (2005), 14; 2290-2296

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Geobacillus tepidamans GS5-97(T); Secondary cell wall polymer (SCWP); Bacterial polysaccharide structure; Glycoforms; Chemical modification

Sažetak
Nuclear magnetic resonance spectroscopic studies of the strain-specific secondary cell wall polymer (SCWP) of the Gram-positive, moderately thermophilic organism Geobacillus tepidamans GS5-97(T) reveal two glycoforms consisting of identical tetrasaccharide repeating units with different chemical modifications of the amide moieties. On the basis of sugar analyses along with 1D and 2D H-1, C-13, N-15 and P-31 NMR spectroscopy at natural isotope abundance, the basic backbone structure of the SCWP was established to be [beta-D-Manp-2, 3-diNAcANH2-(1->6)-alpha-D-Glcp-(1->4)-beta-D-Manp-2, 3-diNAcANH2-(1->3)-alpha-D-GlcpNAc-(1->]6-(1->O)-PO2-(O->6)-MurNAc-, with modifications of the amide groups. In one glycoform, all beta-D-Manp-2, 3-diNAcANH2 (2, 3-diacetamido-2, 3-dideoxy-beta-D-mannopyranuronamide, ManpANH2) residues are substituted with two acetyl groups (glycoform I) at the amide group in position 6 ; in the other glycoform (glycoform II), only one proton of this amide group is substituted by an acetyl group. The ratio between both glycoforms approximates 1:1.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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