Naslov
Determination of Mammillaria gracillis N-glycan patterns by ESI Q-TOF mass spectrometry

Autori
Balen, Biljana ; Zamfir, Alina ; Vakhrushev, Sergey ; Krsnik-Rasol, Marijana ; Peter-Katalinić, Jasna

Izvornik
Croatica chemica acta (0011-1643) 78 (2005); 463-477

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cactaceae; crown-gall tumour; morphogenesis; N-glycans; sialic acid

Sažetak
N-glycans detached from the 42 kDa cellular glycoprotein of in vitro propagated cactus (Mammillaria gracillis Pfeiff.) shoots, callus, hyperhydric regenerants and TW tumour were analysed by mass spectrometry after in-gel deglycosylation of excised protein band. Mixtures of N-glycans were shown by electrospray MS analysis to be highly complex and heterogenous. Only a low amount of high-mannose N-glycans was present, while the majority of structures were of the complex-type N-glycans. In the shoot sample a single N-glycan structure was observed, which appeared to be in good correlation with our previous results. In the callus and tumour tissue similarities of assigned structures were revealed while the highest number of different oligosaccharide structures was found in the hyperhydric regenerant. The presence of sialic acid as a constituent of N-glycans has been indicated by lectin staining, ion exchange chromatography and molecular mapping by mass spectrometry, and could be correlated to the differentiation status of tissues. Results obtained in this study indicate that different morphological levels can be correlated to the N-glycan composition of cellular glycoproteins.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA