Naslov
Cellular antigen stimulation test (CAST) in diagnosis of Hymenoptera venom allergy

Autori
Rudolf, Maja ; Milavec-Puretić, Višnja ; Lipozenčić, Jasna ; Malenica, Branko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Journal of the World Allergy Organization / - Göttingen : Hogrefe & Huber Publishers, 2005

Skup
XIX World Allergy Organization Congress and XXIV Congress of the European Academy of Allergology and Clinical Immunology, World Allergy Congress ,

Mjesto i datum
München, Njemačka, 26.06.2005. - 01.07.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Cellular antigen stimulation test (CAST); Hymenoptera venom allergy

Sažetak
Insect venom very frequently induced allergic reaction. People who have allergy reaction to an insect sting have a 60% chance of similar or worse reaction if stung again.c The history of insect venom allergies may be very suggestive, and it is important to confirm these suspicious with skin testing or blood tests. The aim of this study was to evaluate the usefulness of CAST-ELISA test in diagnosis of allergic reaction mediated by bee and wasp venom. Methods: Cellular antigen stimulation test (CAST) was applied to measure the production of sulfidoleukotriens (sLT) released byreactive cells after in vitro specific allergen cell stimulation. Twenty two patients (7 female and 15 male) with clinical symptoms of hypersensitivity reactions to bee and wasp venom were studied. The reactions types were urticaria and/or angioedema (14 patients) and anaphylaxis (8 patients). Results: Positive CAST test result to bee venom was found in 84% (16/19) of patients and 77% (17/22) of all patients was positive towasp venom. Furthermore, 68% (13/19) of these patients had positive CAST to both venoms. Conclusion: Although this is a smal number of bee and wasp venom senstive patients, we observed high association between positive clinical history and positive CAST test (91% ; 20/22). Detection of allergen-induced sLT thus appears promising for in vitro confirmation of hypersensitivity reactions to bee and wasp venoms.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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