Naslov
Selective inhibition of divergent seryl-tRNA synthetases by serine analogues

Autori
Ahel, Dragana ; Slade, Dea ; Močibob, Marko ; Söll, Dieter ; Weygand-Đurašević, Ivana

Izvornik
FEBS letters (0014-5793) 579 (2005), 20; 4344-4348

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
seryl-tRNA synthetase ; substrate analogue ; selective inhibition ; serinamide ; evolution

Sažetak
Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNASer recognition. In order to determine whether the two types of SerRSs also differ in their recognition of the serine substrate, we compared the sensitivity of the representative methanogenic and bacterial-type SerRSs to serine hydroxamate and two previously unidentified inhibitors, serinamide and serine methyl ester. Our kinetic data showed selective inhibition of the methanogenic SerRS by serinamide, suggesting a lack of mechanistic uniformity in serine recognition between the evolutionarily distinct SerRSs.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA