Naslov
Proscillaridin A immunoreactivity: its purification transport in blood by a specific binding protein and its correlation with blood pressure

Autori
Schneider, Ralf ; Antolović, Roberto ; Kost, Holger ; Sich, Beate ; Kirch, Ulrike ; Tepel, Martin ; Zidek, Walter ; Schoner, Wilhelm

Izvornik
Clinical and experimental hypertension (1064-1963) 20 (1998), 5-6; 593-599

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
endogenous digitalis-like factor; proscillaridin A immunoreactivity; ouabain immunoreactivity; sodium pump; cardiac glycoside binding globulin

Sažetak
A material crossreacting with antibodies against the bufadienolide proscillaridin A and inhibiting the sodium pump was found in human blood plasma. The concentration of the material with a retention time similar to ouabain in a reversed phase HPLC correlated to systolic blood pressure and puls pressure. Affinity purification of this compound from bovine adrenals resulted in the isolation of a compound with molecular mass of 600 Da that was not identical to Ouabain. Consistent with the postulate that endogenous ouabain and prosscilaridin A immunoreactivities may belong to a new class of cardiotonic steroid hormones, a protein of Mr=60 kDa has been found in bovine serum and by affinity-labeling with N-hydroxysuccimidyl digoxigenin-3-O-methylcarbonyl-epsilon-aminocaproate.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA