Pregled bibliografske jedinice broj: 197643
How crystallography can contribute to MALDI-mass spectrometry: intermolecular interaction of crystalline MALDI-matrix and analyte
How crystallography can contribute to MALDI-mass spectrometry: intermolecular interaction of crystalline MALDI-matrix and analyte // Fourteenth Croatian-Slovenian Crystallographic Meeting, Book of Abstracts / Cetina, Mario (ur.).
Zagreb, 2005. (predavanje, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 197643 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
How crystallography can contribute to MALDI-mass spectrometry: intermolecular interaction of crystalline MALDI-matrix and analyte
Autori
Molčanov, Krešimir ; Kojić-Prodić, Biserka ; Zehl, Martin ; Allmaier, Günther
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Fourteenth Croatian-Slovenian Crystallographic Meeting, Book of Abstracts
/ Cetina, Mario - Zagreb, 2005
Skup
Fourteenth Croatian-Slovenian Crystallographic Meeting
Mjesto i datum
Vrsar, Hrvatska, 15.06.2005. - 18.06.2005
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Domaća recenzija
Ključne riječi
MALDI-MS; hydrogen bonding; matrix; crystal morphology
Sažetak
A successful MALDI (Matrix-Assisted Laser Desorption/Ionization) analysis includes the following steps: sample preparation, excitation of sample and disintegration of condensed phase, ionisation of analyte molecules, and at the end, separation of the ions in the spectrometer according to the mass-to-charge ratio and their detection. In spite of exponential development of the mass spectrometry and its wide use in chemistry and proteomics, the question of matrix-analyte interaction in matrix crystals at molecular level has not been completely understood. The goal of our research is to find out how protein molecule incorporates into MALDI-matrix crystals during experiment and to find out the factors influencing matrix-analyte interactions. The purpose this research is to detect undesired effects and to select the favourable organic molecules to be used as matrices. The experiments include 2, 6-dihydroxyacetophenone and 2, 4, 6-trihydroxyacetophenone interacting with avidin. First, the conditions of crystallization of matrices have to be as those during the experiments ; they influence the shape and size of crystals, parameters essential for the matrix-analyte interactions. Here we report the crystal structures of two MALDI-matrices and the morphologies. To visualize MALDI-matrix/analyte interactions atomic force microscopy (FAM) and scanning electron microscopy will be used.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
