Naslov
Changes in catalytic activity of conjugated horse-radish peroxidase

Autori
Časl, Martin, Tino ; Malnar, M ; Mildner, Boris ; Pende, Berislav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biological Chemistry Hoppe-Seyler ; 17th FEBS Meeting, Berlin / Decker, K. ; Stoffel, W. ; Zachau, H. G. - Berlin : New York : Walter de Gruyter, 1986, 369-369

Skup
17th FEBS Meeting, Berlin

Mjesto i datum
Berlin, Njemačka, 24.08.1986. - 29.08.1986

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
horse radish peroxidase; immunoglobulin; chorionic somatomammotropin; catalytic activity

Sažetak
To investigate how different sizes of protein ligands change peroxidase catalytic activity, we labeled IgG and chorionic somatommamotropin (HCS) with horse radish peroxidase (HRP) by two-step glutaraldehyde method. Guiacol and o-phenylendiamidine (OPD) were used as hydrogen donors. Following values were calculated (expressed as conjugated enzyme activity/free enzyme activity): (a)IgG-HRP - Rate-guiacol (Vmax)=0, 23 ; OPD-Rate (Vmax)=0.20 ; compound I formation constant: k1-guicol=0.25 ; k1-OPD=0.22 ; releasing constant k4: guiacol k4=0.23 ; k4-OPD=0.21 ; (b) HCS-HRP conjugate: rate (vmax)guiacol= 0.57 ; Vmax OPD = 0.68 ; compound I formation constant k1-guiacol=0.68 ; k1-OPD=0.75 ; releasing constant k4-guiacol=0.48 ; releasing constant k4-OPD=0.75.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA