Naslov
Nerve growth factor (NGF) induced differentiation of human neuroblastoma cells

Autori
Pavelić, Krešimir ; Spaventi, Šime

Izvornik
International journal of biochemistry (0020-711X) 19 (1987), 12; 1237-1240

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
nerve growth factor (NGF) ; diffenetiation ; human neurofibrosarcoma cells

Sažetak
Nerve growth factor (NGF) induced differentiation of human neuroblastoma cells. The differentiated cells had a relatively high activity of adenylate cyclase and cyclic AMP phosphodiesterase, and a high intracellular level of cyclic AMP. These cells synthesized a higher amount of met5-oenkephalin than undifferentiated cells. Undifferentiated cells bound less met5-enkephalin than differentiated cells. The maximum number of [3H]met5-enkephalin receptor sites per mg of membrane protein increased more in differentiated cells. Previous observations taken together with our results suggests that increased intracellular levels of cyclic AMP after treatment with NGF induced differentiation of human neuroblastoma cells. Reversal of undifferentiated tumor cells into the differentiated changes the capacity of synthesis of met5-enkephalin and its interaction with receptors.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA