Naslov
Antimetabolic activity of L-ascorbic acid in human and animal tumors

Autori
Pavelić, Krešimir ; Kos, Z. ; Spaventi, Šime

Izvornik
International journal of biochemistry (0020-711X) 21 (1989), 8; 931-935

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
L-ascorbic acid ; tumors

Sažetak
The effect of high concentrations of l-ascorbic acid on the growth of some human and animal transformed and non-transformed cell lines has been investigated. Directly implemented into culture of transformed cell lines it decreased [3H]thymidine, [3H]uridine and [3H]leucine incorporation into cells. Vitamin C inhibited DNA synthesis by transformed cells 3–4 times more efficiently than by normal cells. In vivo treatment of athymic nude mice bearing human mammary carcinoma with 500mg/kg l-ascorbic acid for the first 15 days markedly inhibited the growth of tumor cells. As determined by alkaline elution, both DNA strand breaks and DNA cross links were observed in mammary carcinoma cells treated with vitamin C. DNA-DNA and DNA-protein cross links in cells treated with l-ascorbic acid were revealed by the proteinase K assay. Removal of vitamin C caused an immediate onset of spontaneous repair of single or double stranded DNA breaks. If, however, vitamin was reintroduced into cell culture, this spontaneous repair was reversed. Our results indicate an antimetabolic activity of l-ascorbic acid in human and animal transformed cells, probably due to lethal damages in DNA.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA