Naslov
Freshly frozen subsequently AMeX processed breast carcinoma tissue - a new possibility for immunohistochemical detection of c-myc oncoprotein

Autori
Grazio, Simeon ; Frković-Grazio, Snježana ; Čabrijan, Tomislav ; Zjačić-Rotkvić, Vanja ; Goldoni, Vesna ; Pećina-Šlaus, Nives ; Kamenjicki, Elena ; Pavelić, Krešimir

Izvornik
Periodicum biologorum (0031-5362) 94 (1992); 215-220

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
breast carcinoma tissue; c-myc oncoprotein; immunohistochemical detection

Sažetak
Normally, oncoprotein p62c-myc is localized in the cell nucleus. In freshly frozen sections, normal immunohistochemical localization of this antigen is preserved but some morphological details have been hampered by destruction pf cell and tissue arhitecture during cryosectioning. To delineate the distribution and intensity of staining for p62c-myc in tumor cells, we used the monoclonal antibody 155-11C7 raised against a synthetic myc peptide. Our results show that the AmeX procedure in previously frozen tissues resulted reproducible nuclear immunostaining for p62c-myc. This finding is important because tissues often kept frozen for steroid receptor analysis can be also used for immunohistochemical localization of oncoproteins.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA