Naslov
Petide hormone receptors in human placenta

Autori
Mildner, Boris ; Marout, Jasminka ; Sekso, Mladen

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 20th FEBS Meeting / Scientific Committee - Budimpešta : Hnugarian Biochemical Society, 1990, 262-262

Skup
20th Meeting of the Federation of European Biochemical Societies

Mjesto i datum
Budimpešta, Mađarska, 19.08.1990. - 24.08.1990

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
human placenta; insulin; epidermal growth factor; somatomedin C

Sažetak
It is well known that human placental tissue is regulated by a number of hormones. Trophoblast cells on their surface, among other receptors, posses receptors for insulin (INS), epidermal growth factor (EGF) and somatomedin-C (IGF-I). These cells as well as their membrane preparations can be used for ligand-receptor binding studies. Trophoblast cells were prepared from placental tissue, and 50000xg pellets from these cells were used as a "receptor rich material". Ligand-receptor interactions, performed on intact cells and on cell pellets, were studied as a function of ligand bindability to the specific receptor. The influence of pH, temp. and time were also studied. In order to ensure that ligands specifically interact with their receptors, ligand-receptor complexeses were precipitated with specific receptor antibodies. The INS and EGF receptor Mr were determined and receptor Tyr-kinase activity measured. The study of ligand receptor interactions can be a valuable tool in clinical practice for determination of specific hormones, and the study of post-receptor events that occur in the cell.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Kliničke medicinske znanosti

POVEZANOST RADA