Pregled bibliografske jedinice broj: 196758
Isolation and characterization of DNA polymerases from human melanoma tissue
Isolation and characterization of DNA polymerases from human melanoma tissue // Proceedings of the FEBS Special meeting on enzymes / Znanstveni odbor kongresa (ur.).
Zagreb, 1979. (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 196758 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Isolation and characterization of DNA polymerases from human melanoma tissue
Autori
Chandra, Prakash ; Balikcioglu, Salim ; Mildner, Boris
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Proceedings of the FEBS Special meeting on enzymes
/ Znanstveni odbor kongresa - Zagreb, 1979
Skup
FEBS Special meeting on enzymes
Mjesto i datum
Cavtat, Hrvatska, 15.04.1979. - 21.04.1979
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
DNA polymerases; reverse transcriptase; human melanoma
Sažetak
DNA polymerases alpha, -beta, -gamma and reverse transcriptase (RT) from human melanoma tissue were purified by successive column chromatography on DEAE-cellulose and phospho-cellulose. Polymerases-alpha and -gamma were further purified on hydroxylapatite column. Ionic, pH and primer-template specificity were determined for each enzyme. Purification-fold (measured under optimal conditions) for pol.-alpha was 231, for pol.-beta 174, for pol.-gamma 205 and for RT 1188. No terminal deoxynucleotidyl transferase activity was detected. M.W. of enzymes estimated by SDS-gel electrophoresis gave a value for pol.-alpha 160000, pol.-beta 38000, pol.-gamma 100000 and for RT 68000. Serological studies with purified RT were performed to estimate an antigenic resemblance to RT-ases from BEV, RD-114, SiSV, AML, RLV and to RT isolated from human myelofibrotic spleen.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti
Napomena
NEPOTPUN BIBLIOGRAFSKI ZAPIS!!
