Naslov
Bovine adrenals contain, in addition to ouabain, a second inhibitor of the sodium pump

Autori
Schneider, Ralf ; Wray, Victor ; Nimtz, Manfred ; Lehmann, Wolf Dieter ; Kirch, Ulrike ; Antolović, Roberto ; Schoner, Wilhelm

Izvornik
The Journal of biological chemistry (0021-9258) 273 (1998), 2; 784-792

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
high pressure liquid chromatography; ESI; electrospray ionization; mass spectrometry; adrenocorticotropic hormone
(pressure liquid chromatography; ESI; electrospray ionization; mass spectrometry; adrenocorticotropic hormone)

Sažetak
In the search for endogenous cardiac glycosides, two different inhibitors of the sodium pump have been isolated from bovine adrenals. Inhibitor A with a molecular mass of 600 Da and a UV maximum at 250 nm was purified from 16 kg of bovine adrenals. The pure substance (<1 ng) inhibited the sodium pump of human red blood cells with an affinity similar to that of ouabain, yet it cross-reacted with antibodies against the bufadienolide proscillaridin A but not against the cardenolide ouabain. Inhibitor A was slightly more hydrophilic than ouabain on RP-C18 high pressure liquid chromatography. Hence, it showed properties similar to the proscillaridin A immunoreactivity (Sich, B., Kirch, U., Tepel, M., Zideck, W., and Schoner, W. (1996) Hypertension 27, 1073-1078) that increased in humans with systolic blood pressure and pulse pressure. Inhibitor B of the sodium pump with a molecular mass of 584 Da was purified 106-fold from 20 kg of bovine adrenals. It cross-reacted with antibodies against ouabain but not with antibodies against proscillaridin A and inhibited the sodium pump of human and rat red blood cells with the same affinity as ouabain. All other properties, such as the retention time in a C18-reversed phase chromatography, molecular mass determination by electrospray mass spectrometry and fragmentation pattern, and UV and 1H NMR spectroscopic data, were identical to ouabain. Hence, sodium pump inhibitor B from bovine adrenals is the cardenolide ouabain.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA