Naslov
Nested polymerase chain reaction for detection of hepatitis C virus RNA in blood derivatives

Autori
Gall-Trošelj, Koraljka ; Kušić, Borka ; Pećina-Šlaus, Nives ; Pavelić, Krešimir ; Pavelić, Jasminka

Izvornik
European journal of clinical chemistry and clinical biochemistry (0939-4974) 33 (1995), 10; 733-736

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
nested PCR; hepatitis C virus

Sažetak
Testing for the presence of hepatitis C virus (HCV) in blood derivatives used in clinical medicine is of enormous importance to ensure the production of safe preparations. So far, no system has been developed for the isolation and detection of hepatitis C virus from blood derivatives. The recently introduced commercial kit for the detection of hepatitis C virus is designed for the isolation and detection of virus from blood serum. A reliable and reproducible method for the isolation of hepatitis C virus RNA, subsequent reverse transcription and nested polymerase chain reaction (PCR) from blood derivatives is described. Od 17 batches of factor VIII, gama-globulin and anti-tetanus, cytomegalovirus and Varicella-zoster immunoglobulin concentrates, respectively (14 negative for anti HCV and 3 of unknown anti HCV status) five were found positive in RNA-PCR.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA