Naslov
Human chromosome 1 telomere behaviour in Mouse A9 cell line

Autori
Jevtov, Irena ; Matulić, Maja ; Ferenac, Marina ; Škrobot, Nikolina ; Rubelj, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Scientific symposium with international participation 45 years of Molecular biology in Croatia & 50 years of double helix : book of abstracts / Ambriović Ristov, A. ; Brozović, A. - Zagreb : Fakultet strojarstva i brodogradnje Sveučilišta u Zagrebu, 2003, 37-37

Skup
Scientific symposium with international participation 45 years of Molecular biology in Croatia & 50 years of double helix (2 ; 2003)

Mjesto i datum
Zagreb, Hrvatska, 20.11.2003. - 21.11.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
human chromosome 1; A9 cell line

Sažetak
Telomeres are specialized structures at the end of all eukaryotic chromosomes. Their role is to protect and stabilize genomes. They are repetitive sequences that interact with specific proteins forming telomere loop (T-loop) or linear end of the chromosome. It is critical for every single cell organism and immortal cell line to keep telomeres stable because their loss results in chromosomal aberrations, deletions and ultimately death through apoptosis. In order to determine whether telomere conformation is endogenous property of the chromosome or cell proteins background is crucial, we introduced human chromosome 1 in mouse A9 cells. We found A9 cells to have unstable telomeres among which 28% were not labeled in addition to permanently changing karyotype. Also, we followed telomere dynamics of human chromosome 1 as well as mouse chromosomes in this cell line using PNA-FISH and Southern analysis. Surprisingly, introduction of human chromosome 1 resulted in total and average telomere shortening, from ~10 kb to ~3kb, as it was demonstrated by Southern blot analysis, and percentage of unlabeled telomeres increased to 66.5%. All chromosomes, human and mouse followed these changes demonstrating that protein cell background is crucial for telomere conformation. We argue that such effect of human chromosome 1 is due to expression of some human proteins interfering with mouse proteins on telomeres.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA