Pregled bibliografske jedinice broj: 196215
Early-senescing human fibroblasts do not demonstrate accelerated telomere shortening
Early-senescing human fibroblasts do not demonstrate accelerated telomere shortening // European Workshop on Telomerase and Telomeres in cancer and aging (3 ; 2004)
Ladenburg, Njemačka, 2004. (poster, nije recenziran, neobjavljeni rad, znanstveni)
CROSBI ID: 196215 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Early-senescing human fibroblasts do not demonstrate accelerated telomere shortening
Autori
Ferenac, Marina ; Polančec, Denis ; Huzak, Miljenko ; Pereira-Smith, Olivia M. ; Rubelj, Ivica
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni
Skup
European Workshop on Telomerase and Telomeres in cancer and aging (3 ; 2004)
Mjesto i datum
Ladenburg, Njemačka, 21.11.2004. - 23.11.2004
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
telomeres; telomerase; abrupt telomere shortening
Sažetak
Most normal mammalian cell lines demonstrate limited growth capacity due to gradual accumulation of senescent cells in the culture. Senescent cells appear in a stochastic manner, initially at a low incidence but with increasing frequency as the culture accumulates more divisions. Since it has been suggested that this phenomenon is regulated by telomere shortening in human cells, we examined telomere length in the subpopulation of senescent cells present in pre-senescent normal human fibroblast cultures. Senescent cells were separated from young cycling cells by either BrdU selection or DiI staining followed by separation on a high-speed cell sorter. Our results demonstrate that telomeres of early-senescing cells shorten at the same rate and are the same length as those of their cycling counterparts.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
