Pregled bibliografske jedinice broj: 196209
Crystal and molecular structure of the bromo derivative of insulin
Crystal and molecular structure of the bromo derivative of insulin // XIX. Croatian Meeting of Chemists and Chemical Engineers : Knjiga sažetaka
Zagreb: Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Hrvatsko kemijsko drustvo, 2005. str. 137-137 (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 196209 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Crystal and molecular structure of the bromo
derivative of insulin
Autori
Đilović, Ivica ; Matković-Čalogović, Dubravka
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
XIX. Croatian Meeting of Chemists and Chemical Engineers : Knjiga sažetaka
/ - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Hrvatsko kemijsko drustvo, 2005, 137-137
Skup
XIX. Croatian Meeting of Chemists and Chemical Engineers
Mjesto i datum
Opatija, Hrvatska, 24.04.2005. - 27.04.2005
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
bromo derivative of insulin ; X-ray structure
Sažetak
Insulin is a polypeptide hormon which together with glucagon controls level of glucose in the bloodstream [1]. In the rhombohedral crystalline form named 2-Zn insulin it appears as a hexamer with the zinc ion octahedrally coordinated by three His imidazole ligands and three water molecules [2]. The three-dimensional structure of the bromo derivative of insulin [space group R3 (hexagonal setting) ; a = 79.552 Å ; ; c = 73.145 Å ; ; ] has been determined at 1.4 Å ; ; resolution by the molecular replacement method using coordinates of the chloro-derivative. The asymmetric unit contains two TR dimers. Formation of the dimer is stabilized by multiple hydrogen- bonding interactions. The binding of zinc is a major factor in forming and stabilizing the hexamers. Because of the conformational differences between dimers, the zinc binding sites are no longer equivalent. The electron density map reveals presence of statistical disorder of the coordination of the zinc bound on T3. In 50 % of the hexamers, the coordination sphere of the zinc ion is filled (together with three His NE2 three water molecules, while in the other 50 % of the hexamers a bromide ion is the fourth ligand in the tetrahedal coordination sphere. Statistical disorder of the coordination of the zinc ion bound on R3 is not observed, the tetrahedral coordination sphere of the zinc ion is completed by a bromide ion. The X-ray diffraction data were collected at the synchrotron Elettra in Trieste. [1] D. Voet, J. G. Voet, Biochemistry, John Wiley & Sons, Inc., New York, 1995. [2] M. J. Adams, T. L. Blundell, E. J. Dodson, G. G. Dodson, N. M. Vijayan, E. N. Baker, M. M. Harding, D. Hodgkin, B. Rimmer, S. Sheat, Nature 224 (1969) 491-495.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
