Naslov
Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Bloodstream Isolates from Croatia

Autori
Budimir, Ana ; Deurenberg, Ruud H. ; Plečko, Vanda ; Vink, Cornelis ; Kalenić, Smilja ; Stobberingh, Ellen E.

Izvornik
Journal of antimicrobial chemotherapy (0305-7453) 57 (2006), 2; 331-334

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
MRSA; typing; PFGE; SCCmec

Sažetak
A total of 82 hospital-acquired methicillin-resistant S. aureus (HA-MRSA) bloodstream isolates, collected in 2001 and 2002 in Croatia, were investigated by Pulsed-Field Gel Electrophoresis (PFGE), SCCmec typing and Multilocus Sequence Typing (MLST). Furthermore, the presence of PVL and TSST-1 was investigated by real-time PCR. All strains were multi-resistant and were distributed among 19 clonal groups as investigated by PFGE, two of which, H and K, were major clonal groups. Group H harbored 52%, whereas group K harbored 12% of the MRSA isolates. A total of 89% MRSA strains harbored SCCmec type I, and 11% harbored a non-standard SCCmec type III, containing an extra dcs region. One strain harbored a novel SCCmec type ; besides four loci (C, D, E, and F), ccrC, which is characteristic for SCCmec type V, was found. MLST analyses showed the presence of ST111-MRSA-I and ST247-MRSA-I among Croatian MRSA isolates. All isolates were negative for both PVL and TSST-1.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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