Naslov
Effect of Sermion on some enzymatic activities in brain

Autori
Chandra, Prakash ; Paul, Anne ; Mildner, Boris

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
La Ricerca Scientifica Nell'Industria Farmaceutica in Italia ; Risultati e Ruolo Internazionale / - Rim : Farmitalia-Milano, 1975, 585-594

Skup
Simposi Internazionale: La Ricerca scientifica nell'industria farmaceutica in Italia

Mjesto i datum
Rim, Italija, 02.10.1975. - 04.10.1975

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Sermion; mice; brain; macromolecular synthesis

Sažetak
The influence of Sermion on cellular protein synthesis in the mouse brain was studied in vivo, after an intraperitoneal administration of 50 microgram doses. Brain samples analyzed at different intervals after drug treatment have shown a short-term increase of 3H-leucine incorporation into proteins, followed by a gradual decline of label uptake. The latter reached control level about 18 h after the drug administration. The interval of enhanced protein synthesis coincides with the period of Sermion degradation, as was found in experiments with rats (13). Under similar experimental treatments, Sermion had no effect on the biosynthesis of RNA catalyzed by Mg++ ions ; however, a slight stimulation of Mn++ activated RNA biosynthesis by the drug was seen. Mice receiving i.p. injections of 50 micrograms of Sermion showed an increased brain methyltransferase activity ; the maximum stimulation was observed one hour after drug treatment. In another set of experiments the ability of mouse brain preparations to catalyze the methyl group incorporation in the exogenously added transfer RNA (tRNA) was found to decrease with increasing the age of the animals ; the addition of 10-4 M Sermion to the incubation medium enhanced endogenous methylation, especially in the older animals. On the contrary, the ability to methylate the exogenously added heterologous tRNA was not affected by Sermion, irrespective of the age of the animals. In an attempt to understand the physiological relevance of the biochemical effects of Sermion, we have carried out some studies on the effect of this drug on conditioned learning in rats. The preliminary data indicate that, the drug is able to enhance the learning quotients, however, more experiments are in progress to reach conclusive evidence.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Farmacija

POVEZANOST RADA